negative shrna control shctrl (Addgene inc)
Addgene inc is a verified supplier 
96
Structured Review
Addgene inc
negative shrna control shctrl
Negative Shrna Control Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+(shctrl)/shRNA+(Plasmid+%2355783)/pm37607946-252-8-17
Average 96 stars, based on 1414 article reviews
Negative Shrna Control Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+(shctrl)/shRNA+(Plasmid+%2355783)/pm37607946-252-8-17
Average 96 stars, based on 1414 article reviews
negative shrna control shctrl - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal."
Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal.
Journal: Signal transduction and targeted therapy
doi: 10.1038/s41392-023-01545-x
Figure Legend Snippet: Fig. 3 A20 inhibited antitumor immune response in vivo. a The expression of A20 in mice CT26-luc-GFP cells. b The cell proliferation of CT26- luc-GFP cells detected by CCK8 kit, n = 4. c The experimental scheme of the animal study. d The in vivo images of mice tumors with different treatments were detected by the IVIS bioluminescence imaging system, n = 7. e Statistical analysis of total flux from the IVIS bioluminescence images. f The representative images of the metastatic nodes in lung from BALB/C mice. g The survival curve of mice, n = 7. h The experimental scheme of the animal study. i The images of tumors excised from BALB/C mice at the end of experiments, n = 6. j Tumor weights of the four treatment groups, n = 6. k Tumor growth curves of the four treatment groups, n = 6. l The images of tumors excised from BALB/C mice at the end of experiments, n = 8. m Tumor weights of the four treatment groups, n = 8. n Tumor growth curves of the four treatment groups, n = 8. o–v The infiltration of CD3 (+) (X400), CD8 (+) (X400), CD4 (+) (X200), and Granzyme B(+) (X400) T cells detected by immunohistochemical staining. p-value was calculated by one-way ANOVA analysis. Shctrl+IgG, n = 7, Shctrl+αPD-1, n = 5, A20sh4+IgG, n = 7, A20sh4+αPD-1, n = 3. w–y Flow cytometry analysis of CD8 (+) and CD4 ( + ) T cells of mice spleens (n = 4 per group). Data was represented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; n.s. not significant
Techniques Used: In Vivo, Expressing, Imaging, Immunohistochemical staining, Staining, Flow Cytometry
Related Articles
Infection:Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of Pontine Diffuse Midline Glioma (DMG)-K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Negative Control:Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of Pontine Diffuse Midline Glioma (DMG)-K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: ADAM12 promotes gemcitabine resistance by activating EGFR signaling pathway and induces EMT in bladder cancer. Article Snippet: Background Gemcitabine (GEM)-based chemotherapy regimens is widely used in bladder cancer (BC) patients.. However, GEM resistance may occur and result in treatment failure and disease progression.. A disintegrin and metalloprotease 12 (ADAM12) plays a critical role in many cancers. shRNA:Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of Pontine Diffuse Midline Glioma (DMG)-K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block “eat-me” signal Article Snippet: Single-guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal. Article Snippet: Single- guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a Article Title: ADAM12 promotes gemcitabine resistance by activating EGFR signaling pathway and induces EMT in bladder cancer. Article Snippet: Background Gemcitabine (GEM)-based chemotherapy regimens is widely used in bladder cancer (BC) patients.. However, GEM resistance may occur and result in treatment failure and disease progression.. A disintegrin and metalloprotease 12 (ADAM12) plays a critical role in many cancers. Control:Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of Pontine Diffuse Midline Glioma (DMG)-K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block “eat-me” signal Article Snippet: Single-guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal. Article Snippet: Single- guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a Plasmid Preparation:Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Article Title: VRK3 depletion induces cell cycle arrest and metabolic reprogramming of Pontine Diffuse Midline Glioma (DMG)-K27 altered cells Article Snippet: Lentiviral particles were produced in HEK293T cells using psPax2 and pMD2.g second-generation packaging plasmids (Addgene #12260, #12259) with jetPRIME Polyplus transfection reagent, and lentiviral titers were then determined by fluorescence assay. .. GSCs were transduced at a multiplicity of infection (M.O.I.) of 3 with shVRK3-1, shVRK3-4, Clone Assay:Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block “eat-me” signal Article Snippet: Single-guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a Article Title: A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal. Article Snippet: Single- guide RNA (sgRNA) targeting A20 gene were cloned into the LentiCRISPR v2 lentiviral backbone vector (Addgene, Watertown, MA, USA). .. The shRNAs of A20 or STC1 and a |
